Extended Data Fig. 6: Effects of kynurenic acid treatment on glucose metabolism disorders associated with TPN.
From: Total parenteral nutrition impairs glucose metabolism by modifying the gut microbiome
(a) Schematic of kynurenic acid-related experiments. (b) The blood concentration of kynurenic acid at the samples harvest time point. c–d. IPGTTs for the two groups of mice (IPGTTs: P = 0.0361 for 60 mins, P = 0.0283 for 120 mins). (c); area under the curve values for IPGTTs (d). (IPGTTs:). e–f. IPITTs (e); values of area under the curve for IPITTs (f). g–h. Representative histologic images showing deposition of glycogen in mouse hepatocytes (g) and quantification of the average levels of liver glycogen (h). (i) Western blot (left) and semiquantitative analyses (right) of insulin-driven glycogen synthesis signaling (IRS1-Akt-GSK3β) in the liver. (j) Body weight changes of mice. (k) Body composition of different groups of mice. (n = 5 animals over 3 independent experiments for b-k). (l) The serum concentration of kynurenic acid in patients with intestinal failure (n = 69). Data are shown as mean ± SEM (c, e), median (solid line) with 1st (lower dotted line) and 3rd (upper dotted line) quartiles in violin plot (b, d, f, h, i, k, l). P-Values are determined by Kruskal-Wallis test with correction by Dunnett’s t test (b), Mann-Whitney test (c-f, h-l). All statistical tests are two-sided.
