Extended Data Fig. 7: Effects of AhR activation and inactivation on glucose tolerance and insulin sensitivity.
From: Total parenteral nutrition impairs glucose metabolism by modifying the gut microbiome
(a) Body weight changes of mice during the intraperitoneal treatment with CH223191 and IAA (* for Chow+ DMSO vs. Chow+CH223191; # for Chow+ DMSO vs Chow+CH22319 + IAA, P-Value for * were 0.0927, 0.0278, 0.0278, 0.0163, 0.0117, 0.0086, 0.0088, 0.0088, 0.0090, 0.0090, 0.0090 from left to right; P-value for # were 0.0283, 0.0090, 0.0090. 0.0086, 0.0090, 0.0088, 0.0088, 0.0088, 0.0090, 0.0088, 0.0090 from left to right). b–c. Area under the curve values for intraperitoneal glucose tolerance tests (b) and intraperitoneal insulin tolerance tests (c), showing the effects of an inhibitor of AhR (CH223191). (d) Body composition of different groups of mice (P-Values were 3.8E-06, 2.1E-06 from left to right). (n = 5 animals over 3 independent experiments for a-d) (e) Body weight changes of mice during intraperitoneal treatment with CH223191 and FICZ. f–g. Area under the curve values for intraperitoneal glucose tolerance tests (f, P-Values were 5.1E-15, 1.6E-06, 1.3E-08 from top to bottom) and intraperitoneal insulin tolerance tests (g), showing the effects of an agonist of AhR (CH223191). (h) Body composition of different groups of mice (P-Values were 3.1E-14, 1.0E-12, 6.0E-06 between groups from left to right; 7.9E-06 for TPN + DMSO vs TPN + FICZ in fat mass). (n = 8 animals over 3 independent experiments for e-h). Data are shown as mean ± S.D. (a, e), median (solid line) with 1st (lower dotted line) and 3rd (upper dotted line) quartiles in violin plot (b-d, f-h). P-Values are determined by Mann-Whitney test (a, e), Kruskal-Wallis test with correction by Dunnett’s t test (b-d, f-h). All statistical tests are two-sided.
