Extended Data Fig. 6: Pharmacological modulation of lysosomal cholesterol affects the SASP.
From: Lysosomal control of senescence and inflammation through cholesterol partitioning
a, Abundance of the indicated mRNAs analyzed by qRT-PCR (n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). SEN (OIS) and Prob denote oncogene-induced senescence and probucol, respectively. b, Abundance of the indicated mRNAs analyzed by qRT-PCR (n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). Glyb denotes glyburide. c, Pearson’s correlation coefficient for colocalization of cellular cholesterol and lysosome (left, n = 2 biologically independent experiments, 10–20 randomly chosen fields from each experiment, box plots show the 1st and 3rd quartiles; whiskers show the minimum and maximum values, one-way ANOVA test with Tukey’s multiple comparisons test). Abundance of the indicated mRNAs analyzed by qRT-PCR (right, n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). SEN denotes DNA damage-induced senescence. d, Abundance of the indicated mRNAs analyzed by qRT-PCR (mean ± SEM, n = 3 biologically independent samples, one-way ANOVA test with Tukey’s multiple comparisons test).
