Extended Data Fig. 2: Cholesterol metabolism is associated with the SASP during DNA damage-induced and oncogene-induced senescence.
From: Lysosomal control of senescence and inflammation through cholesterol partitioning
a, Enzymatic measurement of cholesterol upon MβCD treatment for 2 days in DMEM supplemented with 15% FBS during DNA damage-induced senescence (n = 3 biologically independent samples, mean ± SEM, two-sided unpaired t test). b, Abundance of the indicated mRNAs analyzed by qRT-PCR upon cholesterol depletion (left, n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). ChDS denotes cholesterol depletion by incubating the cells with DMEM supplemented with 15% charcoal-stripped FBS. Abundance of the indicated proteins analyzed by immunoblotting upon senescence induction in BJ cells (right, n = 2 biologically independent experiments). The cells were starved for amino acids and serum, and then restimulated for the indicated time. c, Abundance of the indicated mRNAs analyzed by qRT-PCR (left, n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). Abundance of the indicated proteins analyzed by immunoblotting (right, n = 3 biologically independent experiments). Lov denotes lovastatin. d-e, Abundance of the indicated mRNAs analyzed by qRT-PCR (mean ± SEM, n = 3 biologically independent samples, one-way ANOVA test with Tukey’s multiple comparisons test). Chol denotes cholesterol stimulation.
