Extended Data Fig. 3: ABCA1 plays a key role in SASP regulation.
From: Lysosomal control of senescence and inflammation through cholesterol partitioning
a, Abundance of the indicated mRNAs analyzed by qRT-PCR upon senescence induction in IMR90 cells expressing the indicated shRNAs (mean ± SEM, n = 6 and 4 biologically independent samples for IL1A/IL1B/IL8 and HMGCR/LRP1/SCARB1, respectively, RM one-way ANOVA test with Tukey’s multiple comparisons test). b, Abundance of the indicated proteins analyzed by immunoblotting upon senescence induction in BJ cells (top, n = 2 biologically independent experiments). Abundance of the indicated mRNAs analyzed by qRT-PCR upon senescence induction in BJ cells (bottom, n = 2 biologically independent samples, mean ± SEM). PRO and SEN denote sham-treated proliferating and DNA damage-induced senescent cells, respectively. C denotes control ABCA1 WT cells. c, Abundance of the indicated proteins analyzed by immunoblotting (left, n = 3 biologically independent experiments). Abundance of the indicated mRNAs analyzed by qRT-PCR upon oncogene-induced senescence (right, n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). d, Abundance of the indicated mRNAs analyzed by qRT-PCR in IMR90 cells stimulated with or without cholesterol during senescence (mean ± SEM, n = 3 biologically independent samples, one-way ANOVA test with Tukey’s multiple comparisons test).
