Extended Data Fig. 9: Additional bioenergetics measurement in uridine or mannose-grown cells.
From: Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions
(a) Representative oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in control and UPP1-expressing K562 cells grown in sugar-free media or in media supplemented with 10 mM of glucose, galactose or uridine, all in the presence of glutamine and dialyzed FBS (n = 30 replicate wells). O: oligomycin. C: CCCP. A: antimycin A. Data are shown as mean ± SD with n > 6 replicate wells. (b) Total consumption of uridine in UACC-257 melanoma cells treated with antimycin A (100 nM) in the same media. Data are shown as mean ± SEM with n = 4. (c) Representative extracellular acidification rate (ECAR) in cells in 10 mM of glucose or mannose and treated as in (a). (d) Schematic representation of de novo pyrimidine synthesis and uridine auxotrophy during OXPHOS inhibition. CoQ: co-enzyme Q. Ox: oxidized. Red: reduced. All experiments included 4mM L-glutamine and 10% dialyzed FBS.
