Extended Data Fig. 7: Effects of Na-l-lactate on tissue gene expression, body weight and food intake. | Nature Metabolism

Extended Data Fig. 7: Effects of Na-l-lactate on tissue gene expression, body weight and food intake.

From: The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions

Extended Data Fig. 7: Effects of Na-l-lactate on tissue gene expression, body weight and food intake.The alternative text for this image may have been generated using AI.

a-e, Gene expression in gastrocnemius muscle and liver from DIO mice treated daily for 16 days with injections of 1 g/kg Na-L-lactate, iso-osmolar NaCl or PBS (mouse study 9 in Methods). a, Expression of selected metabolic genes in gastrocnemius (PBS n = 8 mice, Na-L-lactate n = 12 mice, NaCl n = 8 mice, Mt2 PBS versus Na-L-lactate p = 0.0208, Mt2 Na-L-lactate versus NaCl p = 0.0187). b, Expression of genes involved in hepatic glycogen synthesis (PBS n = 8 mice, Na-L-lactate n = 12 mice, NaCl n = 8 mice). c, Expression of genes involved in hepatic glycogenolysis (PBS n = 8 mice, Na-L-lactate n = 12 mice, NaCl n = 8 mice). d, Expression of genes involved in hepatic glucose transport (Glut1, Glut2) and glycolysis (Hk1, Hk2, Gck, Pfkl, Pgk1, Pkm1, and Pkm2) (PBS n = 8 mice, Na-L-lactate n = 12 mice, NaCl n = 8 mice). e, Expression of genes involved in hepatic gluconeogenesis (PBS n = 8 mice, Na-L-lactate n = 12 mice, NaCl n = 8 mice, Pck1 PBS versus Na-L-lactate p = 0.0230). f-i, Changes in body weight in DIO mice treated with daily injections of 1 g/kg Na-L-lactate or PBS for one week while single-housed in metabolic cages (mouse study 20 in Methods). f, Percentage change in body weight during the study (PBS n = 8 mice, Na-L-lactate n = 8 mice). g, Percentage change in body weight at day 7 (PBS n = 8 mice, Na-L-lactate n = 8 mice, PBS versus Na-L-lactate p = 0.0008). h, Change in absolute body weight during the study (PBS n = 8 mice, Na-L-lactate n = 8 mice). i, Change in absolute body weight at day 7 (PBS n = 8 mice, Na-L-lactate n = 8 mice). j-m, Changes in body weight in DIO mice treated with daily injections of 1 g/kg Na-L-lactate or iso-osmolar NaCl for one week while single-housed in metabolic cages (mouse study 21 in Methods). j, Percentage change in body weight during the study (NaCl n = 8 mice, Na-L-lactate n = 8 mice). k, Percentage change in body weight at day 7 (NaCl n = 8 mice, Na-L-lactate n = 8 mice). l, Change in absolute body weight during the study (NaCl n = 8 mice, Na-L-lactate n = 8 mice). m, Change in absolute body weight at day 7 (NaCl n = 8 mice, Na-L-lactate n = 8 mice). n, Cumulative food intake in response to 1 g/kg Na-L-lactate or PBS (mouse study 20 in Methods) (PBS n = 8 mice, Na-L-lactate n = 8 mice). o, Total food intake after 190 hours (mouse study 20 in Methods) (PBS n = 8 mice, Na-L-lactate n = 8 mice, PBS versus Na-L-lactate p = 0.0116). p, Cumulative food intake in response to 1 g/kg Na-L-lactate or iso-osmolar NaCl (mouse study 21 in Methods) (NaCl n = 8 mice, Na-L-lactate n = 8 mice). q, Total food intake after 190 hours (mouse study 21 in Methods) (NaCl n = 8 mice, Na-L-lactate n = 7 mice). Dashed vertical lines indicate time of injection (n and p). Dark phases are indicated by grey-shaded areas (n and p). Data analyzed by two-way ANOVA with Bonferroni post hoc test (a-e) and by unpaired two-tailed t-test (g, k, o, and q). Data shown as the mean ± SEM; * p < 0.05,*** p < 0.001.

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