Extended Data Fig. 8: Effects of succinate and counterion salts on body weight.
a-b, Lean mice shifted from chow to high-fat diet and with ad libitum access to either tap water, tap water with NaCl, tap water with disodium succinate or tap water with disodium fumarate for 28 days (mouse study 22 in Methods). a, Change in absolute body weight during the study (Tap water n = 10 mice, NaCl n = 10 mice, disodium succinate n = 11 mice, disodium fumarate n = 11 mice). b, Change in absolute body weight at day 28 (Tap water n = 10 mice, NaCl n = 10 mice, disodium succinate n = 11 mice, disodium fumarate n = 11 mice). c-d, DIO mice treated once with an injection of 210 mg/kg MgCl2 or PBS (mouse study 23 in Methods). c, Absolute body weight before (pre = 0 hours) and after (post = 24 hours) the injection (PBS n = 6 mice, MgCl2 n = 6 mice). d, Change in absolute body weight after 24 hours (PBS n = 6 mice, MgCl2 n = 6 mice). e-f, DIO mice treated once with an injection of 60 mg/kg LiCl or PBS (mouse study 24 in Methods). e, Absolute body weight before (pre = 0 hours) and after (post = 24 hours) the injection (PBS n = 6 mice, LiCl n = 6 mice). f, Change in absolute body weight after 24 hours (PBS n = 6 mice, LiCl n = 6 mice). g-h, DIO mice treated once with an injection of 40 mg/kg CaCl2 or isotonic saline (mouse study 25 in Methods). g, Absolute body weight before (pre = 0 hours) and after (post = 24 hours) the injection (isotonic saline n = 7 mice, CaCl2 n = 7 mice). h, Change in absolute body weight after 24 hours (isotonic saline n = 7 mice, CaCl2 n = 7 mice). Data shown as the mean ± SEM.
