Fig. 3: GAPDH oxidation supports proliferation under peroxide stress.
From: The GAPDH redox switch safeguards reductive capacity and enables survival of stressed tumour cells
a, Cell growth, as measured by optical imaging, of WT and mutant cells, either left untreated (left), or seeded in the presence of 100, 150 or 250 µM H2O2 (middle and right). Based on n = 5 technical replicates, representative of n = 3 biological replicates. Dotted lines: standard deviation (s.d.). b, Cell growth, as measured by optical imaging, of WT and mutant cells seeded in the presence of 150 µM H2O2, and then treated with 30 µM of nucleotide (NUC) mixture (left) or 10 mM of NAC (right). Based on n = 5 technical replicates, representative of n = 3 biological replicates. Dotted lines: s.d. c, Cell growth, as measured by optical imaging, of WT and mutant cells seeded with 5 µM of the IMPDH inhibitor merimepodib (VX-497), in the absence (left) or presence (right) of the nucleotide (NUC) mixture (30 µM). Based on n = 5 technical replicates, representative of n = 3 biological replicates. Dotted lines: s.d.
