Extended Data Fig. 4: OTCK307 was carbamylated.a. MS/MS spectrum generated tryptic peptide libraries of mouse liver.
From: Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle
b. Formation of CP-K307 of synthetic OTC K307 peptide, OTC K307R peptide after reacting with 5 mM CP was detected using MS. c-d. Validation of custom-made OTCCP-K307 antibody (abclonal, China). Employing dot-blot assays, the OTCCP-K307 antibody was tested for its reactivity to acetylated and carbamylated OTC K307 peptide (c) and to OTC K307 and OTC K307R peptides after reacting with 5 mM CP (d). e. CP increases intracellular OTCCP-K307 levels. OTCCP-K307 levels of ectopically expressed OTC in HEK293T after treatment with 5 mM CP for 4 h.
