Extended Data Fig. 5: SIRT4 decarbamylates OTCCP-K307.
From: Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle
a. SIRT4 has decarbamylase activity. The formation of decarbamylated OTC peptide from synthetic OTC CP-K307 peptide were detected by MS after the synthetic peptide was incubated each 5 μM recombinant SIRT3, SIRT4 and SIRT5. b. HPLC analysis for the decarbamylation of OTC CP-K307 peptide by SIRT4. Time-dependent production of decarbamylated OTC K307 peptide was monitored (upper) and confirmed by MS/MS analysis (bottom). c-d. OTC interacts with SIRT4. Co-immunoprecipitation analysis was employed to analyze ectopically expressed OTC and SIRT4 in HEK293T (c) and endogenous OTC and SIRT4 in mice hepatocytes (d).
