Extended Data Fig. 7: Deletion of Sirt4 ameliorates hepatic encephalopathy phenotypes.
From: Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle
a. Schematic depicting the experimental setup of HE mice. Wild-type and Sirt4-/- C57 mice were intraperitoneally injected with 30% CCl4 (5 mL/kg body weight, twice a week) for 10 weeks to build the mouse model of HE before assessment. b-c. Food intake (b) and weight (c) were determined for wild-type and Sirt4-/- C57 mice treated with or without CCl4; number of mice = 10. Data are presented as mean ± SEM. d-e. Sirt4 ablation has no impact on hepatic damage induced by CCl4. Total blood bilirubin (d) and blood AST activity (e) were determined in wild-type and Sirt4-/- C57 mice treated or not treated with CCl4 (n = 10 biologically independent samples, d: P = < 0.0001, > 0.05, > 0.05; e: P > 0.05; g: P = < 0.0001, > 0.05, > 0.05).
