Extended Data Fig. 2: BCKDK SM knockout is specific and does not affect body weight or muscle physiology on chow diet.
a–d, Gene expression data via qPCR of Bckdk, Bckdk exon 6 & 7 (deleted region), Bckdha, and Bckdhb, respectively; n = 3 Bckdkl/l (WT) and n = 5 Bckdkl/l-HSA-CreER (Cre). e, Body weights; n = 8 WT and n = 8 Cre. f-j, Western quantification of BCKDK in fasted tissues normalized to loading control; n = 3 WT and n = 3 Cre. k-o, Western quantification of the ratio pBCKDH/BCKDH in fasted tissues; n = 3 WT and n = 3 Cre. p, Western blotting for p-ACLY and ACLY in fasted livers; n = 6 WT and n = 6 Cre. 14-3-3 is the loading control. q, Western quantification of the ratio p-ACLY/ACLY from the blot in p. r, H&E staining of tibialis anterior (TA) muscle. Representative images from n = 4 WT and n = 3 Cre mice. s, Ratio of 3-HIB/valine, and t, Concentration of 3-HIB in the plasma from mice fasted for 5-hr (n = 10 WT and n = 8 Cre) or re-fed for 2-hr after an overnight fast (n = 10 WT and n = 8 Cre). Mice used for these experiments were male, aged 8–10 weeks and fed normal chow. Data are presented as means ± SEM. Comparisons of two groups use two-tailed Student’s t-test with significance defined as: *p < 0.05, **p < 0.01, ***p < 0.001. Exact p-values: a, p = 0.000060. b, p = 0.000020. g, p = 0.008871. h, p = 0.031431. l, p = 0.039537. m, p = 0.015917. s, fasted p = 0.002253, re-fed p = 0.029746. t, re-fed p = 0.023789.
