Extended Data Fig. 3: Identification of murine plasma-derived and AML12 hepatocytes supernatant-derived sEVs.
From: SIRT2 regulates extracellular vesicle-mediated liver–bone communication
a, Electron microscopy images of sEVs isolated from plasma of aged LoxP and SIRT2-KOhep mice (18 months) (scale bar, 100 nm). n = 2 mice. b, Western blot analysis of sEVs protein markers TSG101, HSP70, ALIX in plasma-derived sEVs. n = 3 mice. c, Representative immunofluorescence images show the internalization of PKH26-labeled plasma-derived sEVs (4 μg/ml) (red) in BMDMs (scale bar, 20 µm). n = 2 biologically independent experiments. d, Western blot analysis of SIRT2 expression in the cytoplasm of SIRT2-knockdown AML12 cells. e, Electron microscopy images of sEVs isolated from AML12 hepatocytes supernatant-derived sEVs (scale bar, 100 nm). f, Western blot analysis of sEVs protein markers TSG101, HSP70, ALIX in the sEVs derived from the supernatant control (NC) and SIRT2-knockdown (SIRT2 shRNA) AML12 cells. g, Nanoparticle tracking analysis (NTA) of the sEVs derived from the supernatant NC and SIRT2 shRNA AML12 cells and isolated by ultracentrifugation. h, Representative immunofluorescence images show the internalization of PKH26-labeled sEVs (4 μg/ml) (red) in BMDMs (scale bar, 20 µm). (d-h, n = 1 biologically independent experiment).
