Extended Data Fig. 6: SIRT2-KOhep has no effects on number of osteoclasts and LRG1 protein level in the osteoclast progenitors in young mice.
From: SIRT2 regulates extracellular vesicle-mediated liver–bone communication
a, b, TRAP staining on paraffin-embedded femur sections in young LoxP (n = 7) and SIRT2-KOhep (n = 7) mice (3 months of age) (scale bar, 100 µm) and quantification of Oc.S/BS is shown on the right. One technical replicate of 7 biological replicates for each group. c,d, Represented in situ immunofluorescence images of murine femurs in young mice (scale bar, 50 µm)and quantitation of ratio of LRG1 and CTSK double positive area to CTSK positive area on bone sections of the young LoxP (n = 3) and SIRT2-KOhep (n = 3) mice. One technical replicate of 3 biological replicates for each group. Data are presented as mean ± SD. with biologically individual data points shown. P values were determined by unpaired two-tailed Student’s t-test (b, d).
