Extended Data Fig. 10: Hepatocyte-derived AGK2-sEVs inhibit human osteoclast differentiation.
From: SIRT2 regulates extracellular vesicle-mediated liver–bone communication
a, Western blot analysis of LRG1 protein expression in the sEVs derived from HepG2 cells treated with AGK2 (5 µM). b, Representative TRAP staining images of human PBMCs cultured with RANKL and sEVs(10 μg/ml) derived from the supernatant of HepG2 cells treated with control or AGK2 (Ctrl-sEVs, AGK2-sEVs) (scale bars, 200 µm). c,d, Number and area of multi-nucleated TRAP+ cells. e, The mRNA expression of osteoclast-specific genes measured by real-time PCR. n = 3 biologically independent experiments. Data are presented as mean ± SD. with biologically individual data points shown. P values are determined by unpaired two-tailed Student’s t-test (c-e).
