Extended Data Fig. 10: Sgms1 overexpression attenuated liver injury in Mettl3ΔHep mice.
From: m6A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice
a, Timeline of AAV8-Sgms1 and AAV8-EGFP treatment in Mettl3ΔHep mice. b, qRT-PCR was used to detect the expression of Sgms1 in AAV-EGFP (n = 5) and AAV-Sgms1 (n = 4) livers. c, Representative IHC of Sgms1 in AAV-EGFP and AAV-Sgms1 livers. Scale bar, 20 μm. d, Mouse body weight gain over time post AAV8 injection (n = 4 for each group). e, Liver injury was assessed by serum AST and ALT from mice in (b) (n = 4/5/4). f, Representative liver macroscopy. Scale bar, 1 cm. g, Representative H&E staining of livers from mice in (f). Scale bar, 50 μm. h, Western blot of liver lysates from mice in (b) for indicated proteins. i, Quantification for the indicated proteins in (h) (n = 3). j, qRT-PCR of livers from mice in (b) for indicated genes (n = 4/5/4). k, IHC of cl-Casp3, Cyt-c, CD45 and Ki67 from AAV-EGFP and AAV-Sgms1 livers, and quantification for cl-Casp3 and Ki67 staining (n = 5/4). Scale bar, 20 μm. l, p-eIF2a, MDA, BIP, Epcam and CK19 IHC in livers from Mettl3ΔHep mice injected with AAV-EGFP or AAV-Sgms1. Data are shown in mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by unpaired two-tailed Student’s t test (b, d, e, I, j and k) or by two-way ANOVA statistics (d).
