Extended Data Fig. 1: Deletion of CaMK1D in mice does not affect body size and CaMK1D knockout mice are born without any gross abnormalities.
From: CaMK1D signalling in AgRP neurons promotes ghrelin-mediated food intake
a, Schematic screen of targeting of the CaMK1D locus using Knockout-first targeting strategy and generation of floxed and global knockout mice. For further details see material and methods. b, Validation of targeted ES clone by southern blot. Genomic DNA was digested using BspHI, BstEII, ApalI or EcoRI. The table shows the expected size of bands corresponding to the restriction enzyme used. A probe recognizing the neoR cassette was used. c, Gender distribution of mice and d, Mendelian ratios of born mice with indicated genetoypes. e, Body length and f, Tibia Length measurements of 7-week-old mice with indicated genotypes (CaMK1D+/+ n = 5; CaMK1D−/− n = 7). Data are presented as mean values +/− SEM.
