Extended Data Fig. 4: Tracing the metabolites in mouse dermal fibroblasts (MDFs).
From: Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression
a, The phylogenetic analysis of BCAT1 by MEGA-X. b, p61E residue of BCAT1 is conserved in mammals based MEGA-X analysis. The arrow indicates the E61 residue of BCAT1. c, A flowchart for the generation of Bcat1E61A knock-in mice using the CRISPR/Cas9 system. d, The verification of genome sequence of Bcat1E61A knock-in mice. e and h, The flux model of Valine-13C5 (e) and Leucine -13C6, 15N1 (h) tracing. f, g, The main flux of Valine-13C5 tracing. N = 3. Mean ± SD. Two-tailed t-test. k-p, The main flux of Leucine -13C6, 15N1 tracing. N = 3. Mean ± SD. Two-tailed t-test.
