Extended Data Fig. 7: Cold-induced glutamine synthetase in BAT.
From: Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis
a, qRT–PCR comparing Glul gene expression in BAT for TN and CC. N = 8 mice per condition. Data are mean ± s.e. ***p = 0.0003 by unpaired two-tailed Student’s t-test. b, Western blot of GS in brown adipocytes during adipogenesis. c, Western blot of GS in different tissues from mice at 22 °C. iB, interscapular BAT; sB, subcutaneous BAT; iW, inguinal white fat; pW, perigonadal white fat; LV, liver; Q, quadricep; S, spleen; H, heart; Lu, lung; B, brain; K, kidney. d-g,15N1-labeled glutamine and glutamate abundances in liver (d,e) or serum (f,g) after 15N-ammonia tracer administration. N = 5 mice were used for each time point except TN 15-minute and CC 5-minute N = 4 mice were used. Data are mean ± s.e. h, Full graph of oxygen consumption rate in mature brown adipocytes transfected with control or Glul targeting siRNAs with or without norepinephrine (NE) stimulation. N = 15 biological replicates. Data are mean ± s.e.
