Extended Data Fig. 1: Establishment of AV metabolomics for BAT and hind limb in mice.
From: Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis
a, Schematic of blood vessels used for AV sampling. The Sulzer’s vein (SV) and femoral vein (FV) were used to characterize BAT and hind limb activity. Systemic arterial blood was collected from the left ventricle (LV). Made with BioRender.com. b, Different biological scenarios reflected by AV gradients across BAT. Positive and negative values indicate net release and absorption, while net zero values indicate metabolite bypass (neither uptake nor release). intracellular futile cycling (release equal to uptake) or intercellular cross-exchange between adipocytes and non-adipocytes. c, Heat map shows different metabolite abundances between LV, SV and FV blood collected from mice adapted to mild (22 °C) or severe cold (6 °C). Box 1 highlights metabolites more abundant in BAT-draining blood (SV) than blood from other sites, regardless of temperature, boxes 2-3 highlight metabolites more abundant in BAT-draining blood (SV) or systemic arterial blood (LV) and temperature-sensitive and box 4 highlights metabolites sensitive to temperature across organs. Each column shows an individual mouse.
