Extended Data Fig. 10: Characterisation of LM2 in obesity-induced metabolic disease.
From: Human resident liver myeloid cells protect against metabolic stress in obesity
a, Proportion of LM2 cells among all myeloid cells (live CD45+CD14+HLA-DR+ cells) in livers from lean individuals (n=5), individuals with obesity and IS (n=2) and individuals with obesity and IR (n=4) (lean vs obesity and IS P=0.0193 and lean vs obesity and IR P=0.0063). b, Proportion of LM cell populations among all myeloid cells (live CD45+CD14+HLA-DR+ cells) in livers from individuals with obesity (n=6). c, Quantification of CD68+S100A9+ recruited LM cells from immunofluorescent staining’s of livers from lean individuals (n=5) and individuals with obesity (n=4). d, Individual channels of the representative immunofluorescence image included in Fig. 4d. Scale bar, 50µm. Images are representative of 5 lean individuals and 4 individuals with obesity. e, Representative immunofluorescence images of positive and negative control for the Tunel assay. Images are representative of 5 lean individuals and 4 individuals with obesity for the negative control and one lean individual and 1 individual with obesity for the positive control. Scale bar, 50µm. f, Individual channels of the representative immunofluorescence image included in Fig. 4e. Scale bar, 50µm. Images are representative of 5 lean individuals and 4 individuals with obesity. g, RNA velocity plot displaying proliferating cells and the myeloid cell cluster 1 consisting of resident LM cells and cDC2s coloured by cell population or h, by lean and obese state. Each arrow indicates the direction and progression of transcriptional states. i, Pseudotime analysis comparing differentiation of lean (n=26) and obese (n=13) LM2 cells (P=0.0002). j, Representative immunofluorescence image of liver spheroids stained for lipids using Nile red. Scale bars, 100µm. Images are representative of multiple liver spheroids from 1 hepatocyte donor and 3 NPC donors. Data are presented as mean ± s.e.m. P values were calculated by one-way ANOVA with adjustment for multiple comparisons (a, I) or by two-tailed Mann-Whitney test (c, i). *p < 0.05, **p < 0.01, and ***p < 0.001. IS, insulin sensitivity; IR, insulin resistance; LMs, liver myeloid cells; NPC, non-parenchymal cells; ns, not significant; SM, steatogenic media; ns, not significant.
