Extended Data Fig. 5: SEAP production by DART-engineered HEK-293 cells upon electrostimulation with different types of batteries.
From: An electrogenetic interface to program mammalian gene expression by direct current
a-c, One (a), two (b) and three (c) Duracell or Energizer alkaline AAA batteries were used, each providing around 1.5 volts. d, One Duracell or Energizer lithium button cell CR2032 provides about 3 volts. e-f, Four Duracell or Energizer alkaline AA (e) and AAA (f) batteries provide around 6 volts. g, A Belkin power bank whose universal USB-A 2.4 A port delivers up to 5 volts. h, Apple and Huawei mobile phone chargers. The charger was directly plugged into mains electricity and the charger wires were cut for connection to the electrodes submerged in the culture medium. i, One Duracell or Energizer 12 volt 23 amp alkaline battery. j, Two Duracell or Energizer 9 volt block alkaline batteries. k, Two Duracell or Energizer 12 volt 23 A alkaline batteries. l, Two A220/504 A Exell batteries provide around 30 volts. SEAP levels in supernatants of culture medium were measured at 24 h after electrostimulation for the indicated time. Columns each represent the mean ± SD of the four indicated data points. P values were calculated between stimulated and non-stimulated group.
