Extended Data Fig. 8: Profiling of inflammatory cytokines, blood pH and GTT insulin levels of T1D mice.
From: An electrogenetic interface to program mammalian gene expression by direct current
a-c, IFN-γ (a), IL-6 (b) and TNF-α (c) levels were measured in the serum of non-stimulated and stimulated T1D mice, at the indicated time points after implantation of alginate-encapsulated DCINS cells. d, Blood pH analysis. Blood samples were collected from three groups (wild-type, non-stimulated T1D and stimulated T1D mice) 3 min after electrostimulation with DC 4.5 volts for 10 s. c, The intraperitoneal glucose tolerance test (GTT) was performed after 8 h of fasting by administration of 1.5 g/kg aqueous D-glucose to mice on day 3 after implantation of microencapsulated cells. Blood samples were taken at the indicated time points. Data points represent the mean ± SD; in (a), (b), (c), (e), n = 5; in (d), n = 10; in (d) and (e), the values were normalized to wild-type group. In (a), (b), (c), statistical analysis was performed between stimulated and non-stimulated groups; in (d), statistical analysis was performed between wild-type and T1D group; in (e), statistical analysis was performed between stimulated and non-stimulated groups (blue), and stimulated and wild-type groups (black).
