Fig. 1: Methionine restriction enhances tumour progression in immunocompetent mice.
a, MR increased intestinal tumour growth in Apcmin+/− mice (n = 21 mice per group, two-tailed unpaired Student’s t-test; values are expressed as mean ± s.e.m.). b, Representative images of H&E-stained ileum and colon sections. Arrowheads, colonic tumours. c, MR reduced the survival of Apcmin+/− mice (n = 7 mice on CTRL diet and 6 mice on MR diet, log-rank test). d, MR sensitized C57BL/6 mice to AOM/DSS-induced death. Regular B6 mice were subjected to a modified AOM/DSS CRC procedure as described in Methods (n = 19 CTRL and 20 MR initial mice, log-rank test). e, MR pre-feeding enhanced tumour progression in C57BL/6 mice in an AOM/DSS CRC model (n = 16 mice on CTRL diet and 10 mice on the MR diet, two-tailed unpaired Student’s t-test; values are expressed as mean ± s.e.m.). f, MR pre-feeding enhanced the growth of orthotopically implanted CT26.CL25 cells in Balb/c mice (n = 10 tumours per group, two-tailed unpaired Student’s t-test, one outlier in the control group was removed by >Q3 + 3.0 times the interquartile range (IQR); box-and-whiskers plot, Tukey with whiskers: Q1 minus 1.5 times the IQR to Q3 plus 1.5 times the IQR). Details of statistical tests are in Methods.
