Extended Data Fig. 2: Dietary methionine restriction enhances tumor progression in immunocompetent Apc^min+/− mice by modulating anti-tumor immunity.

a, MR reduces the fraction of CD3⁺CD8⁺ T cells in the blood of Apc^min+/− mice. Representative flow cytometry plots are shown. b, MR has a trend to reduce the fraction of CD3⁺ T cells in intestinal tissues of Apc^min+/− mice (n = 4 mice in each group, two-tailed unpaired Student’s t-test). c, MR from age of one month increases tumor growth in the intestine of Apc^min+/−mice. One-month old Apc^min+/− mice were fed with with CTRL or MR diet for 3 months (n = 10 mice for CTRL diet, 9 mice for MR diet, two-tailed unpaired Student’s t-test). d, MR from age of one month reduces blood T cells abundance in Apc^min+/−mice. Apc^min+/−mice were fed as in (c) and the cell number of indicated cells in the blood was analyzed by FACS (n = 10 mice in each diet, two-tailed unpaired Student’s t-test). e, Correlation between colon tumor burden and blood T cell faction of Apc^min+/− mice fed with CTRL or MR diet for 3 months (n = 10 mice for CTRL diet, 9 mice for MR diet). The Pearson correlation coefficient between the tumor burden in the colon with the blood fraction of indicated T cells was analyzed in Prism. f–g, MR suppresses intestinal tumor growth in Apc^min+/− mice on an immunodeficient background. Apc^min+/− /Rag2^−/− mice were fed with CTRL or MR diet for about 2 months. Their small intestinal tumor number and colon tumor burden were analyzed around 4 months of age (n = 8 mice on the CTRL diet and 9 on the MR diet, two-tailed unpaired Student’s t-test). h, Healthy survival of Apc^min+/− /Rag2^−/− mice under CTRL or MR diet (n = 8 mice on the CTRL diet and 9 on the MR diet, log-rank test). Values are expressed as mean ± s.e.m., except (e and h). Details of statistical tests are included in the Methods.

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