Extended Data Fig. 4: Dietary methionine restriction alters gut microbiota in different immunocompetent mice.
a, MR alters fecal bacterial composition in Apcmin+/− mice. Apcmin+/− mice were fed with a CTRL diet or a MR diet (n = 12 mice on CTRL diet and 11 mice on MR diet, two-tailed unpaired Student’s t-test). The ratio of firmicutes/Bacteroidetes was calculated. b, MR alters the fecal abundance of A. muciniphila, Odoribacter, and Bifidobacterium. c, MR alters fecal bacterial composition in B6 mice. C57BL/6J (B6) donor mice were fed with a CTRL diet or a MR diet (n = 10 mice on CTRL diet and 10 mice on MR diet, two-tailed unpaired Student’s t-test). d, MR reduces firmicutes in the small intestine of B6 mice. C57BL/6J (B6) donor mice were fed with a CTRL diet or a MR diet. Total intestinal DNA samples were analyzed for microbiome using 16S rRNA gene amplicon sequencing as described in Methods (n = 10 mice/group, two-tailed unpaired Student’s t-test). e, MR reduces bacilli while increasing actinobacteria in the small intestine of B6 mice (n = 10 mice/group). Values are expressed as mean ± s.e.m. Details of statistical tests are included in the Methods.
