Extended Data Fig. 6: scRNA-seq analysis of CD45+ immune cells from the small intestine of diet-fed B6 donor or fecal transplanted Apcmin+/− mice.
a, Dot plot showing the expression of marker genes in different function groups of total CD45+ immune cells. Cell population annotation of CD45+ immune cells from CTRL or MR fed B6 donor mice and from Apcmin+/− mice transplanted with fecal solution from CTRL or MR B6 mice. b, Total CD45+ immune cells from the whole small intestine of indicated mice were analyzed by scRNA-seq as described in Methods. c, Heat map showing the expression of marker genes in different sub-groups of T cells. d, Sub T cell group annotation. e, Dietary methionine restriction and MR diet-trained fecal microbiota reduces the fraction of several subgroups of intestinal CD8+ T cells. f, Representative FACS plots for in vitro activation of T cells by fecal microbes from control diet or MR diet fed B6 mice.
