Extended Data Fig. 2: Decreased glucose abundance in tumour epithelium. | Nature Metabolism

Extended Data Fig. 2: Decreased glucose abundance in tumour epithelium.

From: Metabolic profiling stratifies colorectal cancer and reveals adenosylhomocysteinase as a therapeutic target

Extended Data Fig. 2: Decreased glucose abundance in tumour epithelium.The alt text for this image may have been generated using AI.

(A) H&E of distal colon tissue with an Apc-deficient tumour [same H&E as shown in Fig. 2B and Extended Data Fig. 3A; N. Adj.: normal adjacent tissue; S: stroma; T: tumour tissue]. Tissues analysed for n = 4 animals; n = 1 shown. Scale bar: 1 mm. (B) DESI-MSI showed decreased abundance of an ion with m/z 215.03 in the tumour epithelial compartment of APC tumours. [N. Adj.: normal adjacent tissue; S: stroma; T: tumour tissue]. Database search (https://hmdb.ca) suggested that this could be assigned as glucose [M+Cl]−. A standard solution of glucose was mixed with chlorinated dopamine to obtain chlorinated glucose for comparison. (C) DESI tandem mass spectrometry showed that the fragmentation of the ion of interest produced ions matched to the fragmentation pattern of the chlorinated standard, further supporting the ID of this metabolite of interest. All highlighted m/z of fragments and precursor ions on the mass spectra are present in both standard and sample.

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