Extended Data Fig. 10: Diabetes-related molecular changes of β-cells show similarities and differences across dysfunctional states and translate to an external mouse dataset.
From: Delineating mouse β-cell identity during lifetime and in diabetes with a single cell atlas
(a) Design of DGE analysis showing original conditions in each used dataset (dataset 8-16wNOD for NOD DGE, datasets db/db and mSTZ for db/db+mSTZ DGE) and axis used for fitting the DGE model. For NOD we also show expression of a known T1D marker B2m. (b) Translation of diabetes model DEG groups (T1D NOD, T2D db/db+mSTZ) to external human and mouse datasets, indicated as normalized activity of gene groups in T1D or T2D (in mice STZ-treated) and healthy samples. Plot titles contain information on species (hs - human, mm - mouse), dataset and number of cells in healthy (H) and diabetic (D) groups. Encircled are gene groups that translate to the external mouse dataset. (c) Expression of genes upregulated in diabetic NOD or db/db+mSTZ cells shown across fine β-cell states and embryonic cell types as annotated in the original study. Cell color annotations are based on healthy and developmental conditions. (d) Overlap between NOD and db/db+mSTZ DEG groups as a ratio of the smaller group.
