Extended Data Fig. 1: Identification of potential regulators of neurotoxic reactive astrocytes via bulk RNA-seq analysis. (Related to Fig. 1).

a, Volcano plot and Venn diagram showing the RNA-seq analysis of 36 upregulated regulators in neurotoxic reactive astrocytes. b, Volcano plot and Venn diagram showing the RNA-seq analysis of 69 downregulated regulators in neurotoxic reactive astrocytes. The DESeq2 R package, along with the Wald test, was utilized to identify differentially expressed genes (DEGs). Genes were categorized as upregulated DEGs when they exhibited a p-value < 0.05 and a log2Foldchange > 0. Conversely, genes were classified as downregulated DEGs when they displayed a p-value < 0.05 and a log2Foldchange < 0. Genes with a p-value ≥ 0.05 were considered stable. Schematic illustrations were created with BioRender.com.