Extended Data Fig. 8: Gain- and loss-of-function of PGC-1α affects DNA methylation events.
From: Molecular control of endurance training adaptation in male mouse skeletal muscle
a, Bar Venn diagram of differentially methylated regions (DMRs) of an unperturbed trained WT (dark gray) and mKO (dark blue) muscle. The common DMR are striped (gray and blue). b, Venn diagram of DMRs of an unperturbed trained mKO muscle (open circle) and differentially expressed genes (DEG) after acute maximal exercise in trained mKO muscle (blue circle). c, Bar Venn diagram of the DMRs that are associated with acute gene expression changes (overlap Fig. 3g and panel b of this figure) of WT (dark gray) and mKO (dark blue) animals. d, All functional annotation clusters of the overlap of panel b (n = 110) with an enrichment score >2. e, Trajectories of transcription factors in untrained (light blue) and trained (blue) mKO mice that are differentially methylated after training. f, Bar Venn diagram of DMRs of an untrained WT or mKO muscle after an acute bout of maximal exercise (light gray or blue, respectively; 0 h and 4 h time point merged) and those of an unperturbed trained WT or mKO muscle (dark gay or blue, respectively). The common DMRs are striped (gray and blue). g-h, Venn diagram of WT (g) and mKO muscle (h) depicting all DMRs in untrained muscle after an acute bout of maximal exercise (open circles) and DEGs upon an acute bout of maximal exercise (colored circle). i, Number of DMRs in untrained sedentary mTG muscle (hypermethylated = solid bar; hypomethylated = open bar) compared to untrained sedentary WT muscle. j, Bar Venn diagram of DMRs of an unperturbed trained WT (dark gray) and untrained mTG (pink) muscle. The common DMRs are striped (gray and pink). k, Venn diagram of DMRs (open circle) and DEG (blue circle) of an untrained sedentary mTG muscle. l, Venn diagrams of all up- and downregulated genes in untrained sedentary mTG muscle (pink) and after an acute bout of exercise in untrained (light orange or blue) and trained (darker orange and blue) muscle. m, Top 3 functional annotation clusters of the up- (orange) and downregulated (blue) proteins in sedentary mTG muscle compared to sedentary WT. Data from 5 biological replicates. Data represent means ± SEM. Statistics of proteomics data were performed using empirical Bayes moderated t-statistics as implemented in the R/Bioconductor limma package and for RNA-seq data with the CLC genomics workbench software. Exact FDR-values of RNA-seq data are displayed in the Source Data file. * indicates difference to Ctrl (pre-exercise condition); *<0.05; **<0.01; ***<0.001. See also Fig. 6; Supplementary Tables 1, 2, 6, 7.
