Fig. 2: Qualitative transcriptional response to exercise depends on training status.
From: Molecular control of endurance training adaptation in male mouse skeletal muscle
a, Schematic representation of the experimental setup (illustration was created using BioRender.com with permission). b, Number of genes differentially expressed immediately (0 h), 4, 6 and 8 h after an acute bout of exhaustion exercise (cut-off: FDR < 0.05; log2(FC) ± 0.6) in untrained and trained muscle. c, Venn diagram of all significantly up- (orange) and downregulated (blue) genes (all timepoints merged) in untrained (light colour, dashed line) and trained (dark colour, solid line) muscle. d, Dot plot of all functional annotation clusters of up- (orange) and downregulated (blue) genes in untrained and trained muscle post-exercise, as well as unperturbed trained muscle with an enrichment score >2. e, Examples of gene trajectories in untrained (light grey) and trained (dark grey) muscle involved in axon guidance. f, Motif activities from ISMARA and expression changes of a predicted target gene that show an opposite regulation in untrained and trained muscle. The data are from five biological replicates and present mean ± s.e.m. Statistics of RNA-seq data were performed using the CLC Genomics Workbench Software. Exact FDR values of RNA-seq data and z-scores of ISMARA data are displayed in Source data. The asterisk indicates difference to Ctrl (pre-exercise condition): *P < 0.05 (for motif activity: *z-score > 1.96); **P < 0.01; ***P < 0.001 (Extended Data Figs. 2–4 and Supplementary Tables 3 and 4).
