Extended Data Fig. 5: Over-expression of Wdr6 increases liver TAG contents, related to Fig. 2.
From: Upregulation of WDR6 drives hepatic de novo lipogenesis in insulin resistance in mice
8-week-old male C57BL/6J mice were injected Ad-WDR6 or Ad-GFP three times with 5-day intervals via tail vein. a, Western blots of WDR6 protein levels of liver, fat and muscle tissue. β-Actin or GAPDH served as a loading control. b-e, Serum FPG levels, TAG levels, serum insulin levels and HOMA-IR index. f, Liver B-Mode ultrasound imaging (left, the area circled in red represents the kidney), calculated average intensity (middle) and hepatic/ renal ratio (right). g, TAG contents in liver tissue, the concentration was normalized to the protein content of the same sample. h, Representative ORO staining of liver sections. Scale bars, 100 μm. i, Serum ALT and AST levels of each group. For (a), n = 3 biologically independent mice per group. (b-e), (g), (i), n = 11 biologically independent mice per group. (f), n = 4 biologically independent mice per group. Data in (a-b), (e-g) and (i) are presented as mean ± SD, determined by two-sided Student’s t-test. Data in (c) are presented as mean ± SD, determined by unpaired two-sided Mann-Whitney test. * P < 0.05, ** P < 0.01.
