Extended Data Fig. 6: Liver WDR6 affects DNL related components, and the abundance of FFAs of TAG side chain, related to Fig. 3.
From: Upregulation of WDR6 drives hepatic de novo lipogenesis in insulin resistance in mice
a. Amount of lipid species in liver tissue of shNC and shWDR6 mice after feeding with CD or HFD, shown as μmol/g. b. qPCR of representative genes of liver glucose and TAG oxidation pathways of shNC and shWDR6 mice after feeding with the CD or HFD. Actb serves as a normalization control. c. Western blots of representative genes of liver TAG metabolism pathways of shNC and shWDR6 mice under the CD or HFD state. β-Actin serves as a loading control. d. A schematic diagram illustrating the de novo synthesis of fatty acids. e. Heat map of fatty acids species of TAG in liver tissues of shNC and shWDR6 mice after feeding with CD or HFD. For (a-c), (e) n = 4 biologically independent mice per group. Data in (a-c) are presented as mean ± SD, determined by two-way ANOVA and Tukey’s multiple-comparisons test. * P < 0.05, ** P < 0.01.
