Extended Data Fig. 4: Faecal wash flushing preserves tissue cells and lead to minor cell shedding.
From: The cellular states and fates of shed intestinal cells
a. Schematic outline of the procedure. Created with BioRender.com b. Tissue cells after in-vivo Hoechst staining showing robust labeling of tissue cells. Scale bar 100 μm. image is representative of 3 independent experiments. c-d. FACS analysis of cells depicting gating strategy to exclude debris, dead cells, and fractionation according to the level of Hoechst 33342 uptake (dubbed as Hoechst positive [H+] and Hoechst negative [H−]) in tissue dissociated cells and in two faecal wash samples. e. Quantification of fraction of Hoechst positive and Hoechst negative cells in the samples indicated in d. f. Preservation of mucus and tissue morphology by Alcian blue and H&E staining respectively. Scale bar 100 μm. images are representative of 2 independent experiments.
