Fig. 1: Pyruvate consumption by MB neurons increases upon memory formation.
From: Glycolysis-derived alanine from glia fuels neuronal mitochondria for memory in Drosophila
a, Mpc1 knockdown (KD) in adult MB neurons impaired memory after single-cycle (1×) training (n = 12, F2,33 = 11.40, P = 0.00017) and spaced training (n = 12, F2,33 = 19.36, P = 3.10−6), but did not affect memory after single-cycle training followed by cold shock (n = 12, F2,33 = 0.83, P = 0.45) or massed training (n = 12, F2,33 = 0.36, P = 0.70). b, The pyruvate sensor Pyronic was expressed in adult MB neurons. The two images show the mTFP and Venus channels. The dashed lines delimit bundles of MB neuron axons, namely the vertical lobes, where the pyruvate FRET signal was quantified. Scale bar, 50 µm (Supplementary Video 1). c, Single-cycle training elicited a faster pyruvate accumulation in MB neuron axons following sodium azide application (5 mM) compared to non-associative unpaired training (n = 14 (control); n = 17 (1×), t29 = 3.39, P = 0.002). d, PDHE1β knockdown in adult MB neurons impaired memory after single-cycle training (n = 10, F2,27 = 5.08, P = 0.01) and spaced training (n = 14 (blue and gray bars); n = 15 (white bar), F2,40 = 6.25, P = 0.004), but did not affect memory after single-cycle training followed by cold shock (n = 10, F2,27 = 1.91, P = 0.17) or massed training (n = 12, F2,33 = 0.27, P = 0.76). All data are presented as mean ± s.e.m. Asterisks (*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant, P > 0.05) illustrate the significance level of a two-sided t-test or of the least significant pairwise comparison following one-way analysis of variance (ANOVA).
