Extended Data Fig. 5: Behavioral controls of Hrm, Mct1, Chk and Sln KD in MB neurons (related to Fig. 3).
From: Glycolysis-derived alanine from glia fuels neuronal mitochondria for memory in Drosophila
a. Hrm knockdown (KD) in adult cortex glia did not affect memory after single-cycle training (RNAi GD1807: n = 9, F2,24 = 2.13, P = 0.14; RNAi HMC03642: n = 10, F2,27 = 2.88, P = 0.07) or single-cycle training followed by cold shock (RNAi GD1807: n = 9, F2,24 = 1.25, P = 0.30; RNAi HMC03642: n = 10, F2,27 = 1.67, P = 0.21). b. Mct1 KD in adult cortex glia did not affect memory after single-cycle training (n = 12, F2,33 = 0.36, P = 0.70) or single-cycle training followed by cold shock (n = 12, F2,33 = 0.49, P = 0.62). c. Chaski KD in adult cortex glia did not affect memory after single-cycle training (n = 12, F2,33 = 0.25, P = 0.78) or single-cycle training followed by cold shock (n = 12, F2,33 = 0.02, P = 0.98). d. Hrm KD in adult MB neurons did not affect memory after single-cycle training (RNAi GD1807: n = 8, F2,21 = 2.31, P = 0.12; RNAi HMC03642: n = 8, F2,21 = 1.30, P = 0.29) or single-cycle training followed by cold shock (RNAi GD1807: n = 8, F2,21 = 0.25, P = 0.78; RNAi HMC03642: n = 8, F2,21 = 0.79, P = 0.47). e. Mct1 KD in adult MB neurons did not affect memory after single-cycle training (n = 8, F2,21 = 0.23, P = 0.79) or single-cycle training followed by cold shock (n = 8, F2,21 = 0.86, P = 0.44). f. Sln KD in adult MB neurons did not affect memory after single-cycle training (RNAi KK104306: n = 14, F2,39 = 1.16, P = 0.32; RNAi GD1940: n = 12, F2,33 = 2.56, P = 0.09) or single-cycle training followed by cold shock (RNAi KK104306: n = 14, F2,39 = 0.11, P = 0.89; RNAi GD1940: n = 12, F2,33 = 0.09, P = 0.91). All data are presented as mean ± SEM. Asterisks (*P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant, P > 0.05) illustrate the significance level of the least significant pairwise comparison following 1-way ANOVA.
