Fig. 2: Pantothenic acid correlates with MYC expression in mammary tumors.
From: Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer
a, Post-DEFFI fluorescent microscopy of WMhigh, WMlow and WMmix tumors shows clonal distribution (note, this is the same image as Fig. 1f, repeated for illustrative purposes) (top). Single-ion DEFFI image of PA (n = 3 for each tumor type) (bottom). b, LC–MS analysis of WM tumors (10 mg dry tissue from each sample was taken for the extraction) shows significant increase of PA in WMhigh tumors (WMhigh versus WMlow, P = 0.00038; WMhigh versus WMmix, P = 0.0019). c, LC–MS analysis of free CoA-SH in WM tumors shows an increase in WMhigh tumors (WMhigh, n = 4; WMlow, n = 4; WMmix, n = 8 tumors from independent animals; WMhigh versus WMlow, P = 0.0313; WMhigh versus WMmix, P = 7.68 × 10−5) (b,c). d, Correlative DEFFI and IHC staining showing the distribution of PA in relation to MYC staining in human PDXs (n = 2 biological replicates for each PDX). e, Correlative DEFFI and IHC staining showing the distribution of PA in relation to MYC staining in representative human core biopsy, showing association of MYC with PA (n = 12 core biopsies). f, Workflow for correlative fluorescence microscopy, EM and NanoSIMS analysis. g, Correlative fluorescence microscopy, EM and NanoSIMS analysis shows 15N derived from 15N-PA infusions predominantly localizing in MYC-high cells, with subcellular localization in mitochondria and nucleoli. h, Cell-wise quantification of 15N/14N ratios in the WMhigh, WMlow areas shows increased amounts of PA-derived labeled 15N in WMhigh cells. For NanoSIMS 34 WMhigh and 32 WMlow individual cells were analyzed from one NanoSIMS run (P = 2.91 × 10−9). All box-and-whisker plots represent the following: line, median; box, IQR; whiskers, 1.5 × IQR limited by largest/smallest NEV. Significance was calculated with an unpaired two-tailed t-test. P values are represented by *<0.05, ** 0.001, ***<0.0001, ****<0.00001.
