Extended Data Fig. 3: N2 activity is detected in thyrocytes.
From: Notch signaling in thyrocytes is essential for adult thyroid function and mammalian homeostasis
A. Representative double immunofluorescent detection of gamma-secretase-cleaved (active) NICD1* plus PAX8 or NICD1* plus CALCITONIN in thyroid sections of control mice (n = 5). 40X objective; n = 5 mice. B. Representative images of immunohistochemistry or -fluorescent detection of NICD1*, NICD1* plus the endothelial cell marker ENDOMUCIN, NOTCH2 (N2), and cleaved (active) NICD2*. 40X objective; scale bars = 100μm; n = 5 mice. C. Representative image of dual staining using amplified in situ hybridization (RNAscope) and immunostaining to detect Notch2 mRNA (red, Opal 690) and NKX2-1 protein (yellow, Opal 570), respectively. n = 3 mice. White arrows: thyrocytes co-stained for Notch2 mRNA and NKX2-1 protein; orange arrows: thyrocytes negative for NKX2-1 protein with low or no Notch2 mRNA. Scale bars = 50 μm.
