Extended Data Fig. 2: Supporting data relating to Fig. 2.
a,b, Pre-experimental (a) bodyweight and (b) cumulative 3-day food intake of chow (n = 64) and HF (n = 27) rats with USI cannulation before refeeding experiments. c,d, Pre-experimental (c) bodyweight and (d) cumulative 3-day food intake of USI chow (n = 36) ad HF (n = 54) USI rats before IVGTT. e,f, Pre-experimental (e) bodyweight and (f) cumulative 3-day food intake of chow (n = 27) and HF (n = 37) USI rats with USI lentiviral (LV) injections before IVGTT studies. g-i, Plasma glucose levels during IVGTT (inset: AUC) of HF rats (g) with USI shMM receiving USI Sal (n = 5) or Cas (n = 7), or (h) no viral injection receiving 4AMBA+Sal (n = 8) or 4AMBA+Cas (n = 8), or (i) with USI shPepT1 receiving USI Sal (n = 7) or Cas (n = 5). j,k, Experimental timeline for collection of blood collection for gut hormone assay in the context of (j) fasting-refeeding or (k) IVGTT studies. l, Plasma levels of GLP1, GIP, and PYY receiving USI Sal (n= 7,6,7) or Cas (n = 8,7,8). *p < 0.05, **p < 0.01 determined by two-tailed t-test or multiple t-tests for IVGTT (g-i). Sal, saline; Cas, casein; 4AMBA, 4-aminobenzoicacid. Data are presented as mean ± s.e.m.
