Extended Data Fig. 4: Oxygen consumption rate (OCR) and energy expenditure.
From: p21-activated kinase 4 counteracts PKA-dependent lipolysis by phosphorylating FABP4 and HSL
All experimental procedures were the same as those described in Fig. 3’s legend. a-c. Indirect calorimetry was performed using an 8-chamber Oxymax system. The mice were acclimatized to cages for 24 h, and data were collected for an additional 48 h to determine the 24-hour O2 consumption rates (VO2), CO2 production rates (VCO2), and average energy expenditure (EE) in mice (n = 5 for NCD WT, NCD KO and HFD WT, n = 6 for HFD KO). d. Oxygen consumption rate was measured using a Seahorse XF analyser in isolated adipocytes. Basal respiration, respiration related to ATP production (uncoupled, difference between OCR before and after 1 µM oligomycin injection), and maximal respiration (difference between OCR after 1 µM FCCP and 0.5 µM rotenone/antimycin A injection) were determined (n = 12). *p < 0.05 versus WT. Data are representative of at least three independent experiments. Values are mean ± SD. ANCOVA analysis (a-c) and unpaired two-tailed t-test between two groups (d) were conducted for statistical analyses. Source data are provided as a Source Data file. MR, metabolic rate.
