Extended Data Fig. 9: Relationship between PAK4 expression in visceral fat and obesity parameters in non-diabetic humans.
From: p21-activated kinase 4 counteracts PKA-dependent lipolysis by phosphorylating FABP4 and HSL
a, b. Scatter plot of adipose PAK4 protein and mRNA levels and metabolic parameters. The coefficient of determination in two sided distributions was used to compare the association of PAK4 expression with metabolic parameters (n = 88). c. Western blot analysis of PAK4, p-HSL-S565, and p-FABP4-T126 in primary visceral adipocytes isolated from lean (BMI < 25), overweight (25 ≤ BMI < 30), and obese (BMI ≥ 30) cohorts (n = 10). d. After PAK4 silencing or overexpression in human adipocytes, the proteins involved in mitochondrial dynamics were analyzed by Western blotting. Data are representative of at least three independent experiments. Values are mean ± SD. One-way ANOVA followed by Bonferroni’s post hoc analysis (c) was conducted for statistical analyses. Source data are provided as a Source Data file. PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; NRF1, nuclear respiratory factor 1; DRP1, dynamin-related protein 1; Fis1, fission 1.
