Extended Data Fig. 4: Repeated perfusion-stimulated exocytosis in mouse islets.
a, A schematic illustration of the perfusion apparatus and corresponding experimental procedure. Created with BioRender.com. b, Representative illustrations of insulin secretion in male and female islets under continuous perfusion stimulation. c, Heatmaps showcasing the β-cell Ca2+ dynamics within male and female islets undergoing intermittent 18.2 mM glucose stimulations. The color-bar indicates the fluorescent intensity within the islets. Each row represents individual β-cells within a singular islet. The x-axis represents the timeframe subsequent to glucose stimulation. d. Time-dependent fusion events in male and female islets triggered by recurring stimulation with 18.2 mM glucose. Male, n = 6 islets from 4 mice; female, n = 5 islets from 4 mice. Data was expressed as mean ± s.e.m. e. Correlations between the secretory capacity observed during two separate glucose stimulations. Scale bar = 10 μm.
