Fig. 7: PCYT2 and pEtN modulation regulate the senescence metabolic reprogramming.
a, FC of pEtN:CDP-Etn ratio in WI38 fibroblasts infected with an adenovirus driving the expression of a control shRNA (shCtrl) or an shRNA targeting the PCYT2 mRNA (shPCYT2) for 7 days, relative to the value of non-infected cells (Prolif.) b, Representative images (left) and percentage (right) of SABG-positive WI38 fibroblasts treated as in a. The percentage is also reported for non-infected proliferating cells. Scale bars, 20 µm. c, mRNA levels of senescence markers scored by RT–qPCR in WI38 fibroblasts treated as in a. n = 6 biologically independent experiments. Data are presented as mean ± s.d. Indicated P values were calculated using an unpaired two-sided Student’s t-test. d, Representative images of DAPI and LipidTox staining of WI38 fibroblasts infected with an adenovirus driving the expression of a control shRNA (shCtrl) or an shRNA targeting the PCYT2 mRNA (shPCYT2) for 7 days. The experiment was repeated independently three times with similar results. e, Heat map of the indicated mRNA levels as measured by RT–qPCR in WI38 fibroblasts proliferating (Prolif.) or subjected to Ras induction and infected with adenoviruses overexpressing GFP, PCYT2 or ETNPPL for 7 days (n = 3). P values (unpaired two-sided Student’s t-test) in gene expression between RAS OIS + GFP and RAS OIS + PCYT2 or between RAS OIS + GFP and RAS OIS + ETNPPL are indicated. f, FC of pEtN:CDP-Etn ratio in WI38 fibroblasts treated as in e, normalized to the value of non-infected cells (Prolif.). For a,b,f, bars represent the means of three biological replicates ± s.d. Indicated P values were calculated using an unpaired two-sided Student’s t-test.
