Extended Data Fig. 8: Female mice data and extended data for glucose homeostasis assessment.
From: Reciprocal activity of AgRP and POMC neurons governs coordinated control of feeding and metabolism
a, Insulin tolerance test (ITT) performed in ad-libitum fed CNO-treated female mice for 1h before insulin injections. b, Glucose levels during ITT experiment relative to basal glucose levels prior to insulin injection. Letters indicate statistical significance between experimental groups: (a) AgRP-Gq vs. control, **** P < 0.001 15 min, ***P = 0.0001 30 min; (b) POMC-Gi vs. control, *P = 0.0131 60 min; and (c) AgRP-Gq;POMC-Gi vs. control, *P = 0.0133 30 min, *P = 0.0224 60 min. c, Glucose tolerance test (GTT) in 16h fasted CNO-treated female mice for 1h before glucose injection. d, Delta glucose levels in 60 min after CNO injection in female mice in ad libitum fed or 16h fast condition. e, Complete curves of glucose levels during ITT in ad libitum fed male mice. *P = 0.006815min and *P = 0.004930 min, AgRP-Gq vs. control. f, Glucose levels during ITT experiment relative to basal glucose levels prior to insulin injection. (a) AgRP-Gq vs. control, **P = 0.0076 15 min and **P = 0.0030 30 min; (b) POMC-Gi vs. control, *P = 0.041 60 min. g, Complete glucose levels curves during ITT protocol in ad libitum fed male mice treated with vehicle prior to insulin injection. h, Complete curves of glucose levels during an GTT protocol in 16h fasted CNO-treated for 1h male mice. (b) POMC-Gq vs. control, *P = 0.013 60 min, **P = 0.0034 90 min and ** P = 0.006 120 min; (c) AgRP-Gq;POMC-Gi vs. control, *P = 0.0107 90 min and * P = 0.0263 at 120 min. i, Glucose excursion induced by vehicle injection in ad libitum fed male mice j, Glucose excursion induced by CNO injection in ad libitum fed male mice. (a) AgRP-Gq vs. control, *P = 0.34 180 min; (b) POMC-Gi vs. control, **P = 0.0013 60 min, *P = 0.022 180 min; and (c) AgRP-Gq;POMC-Gi vs. control, **P = 0.0083 180 min. k, Changes in glucose levels in 60 min intervals after CNO injection in ad libitum fed male mice. ***P = 0.0008 POMC-Gi vs. control. l, Expression of gluconeogenic enzymes G6P and Pck1 from liver extracts from CNO-treated male mice for 1h. **P = 0.0055 POMC-Gi vs. control, *P = 0.0139 AgRP-Gq;POMC-Gi vs. control. m, Glucose levels during ITT in ad libitum fed Control-GiPVH (grey lines) and Npy1R-GiPVH (orange lines) male mice treated with vehicle (dashed lines) or CNO (solid lines) 1h prior to insulin injection. n, Glucose excursion during GTT protocol in 16h fasted male Control-Gi and Npy1R-Gi mice treated with vehicle (dashed lines) or CNO (solid lines). Data are indicated as mean ± s.e.m. from the following biological replicates for Control, AgRP-Gq, POMC-Gi and AgRP-Gq;POMC-Gi groups, respectively: a, b (n = 17, 13, 15, 13 female mice), c (n = 16, 15, 14, 12 female mice), d (n = 10, 13, 10, 12 female mice), e, f (n = 20, 12, 15, 13 male mice), g (n = 5, 4, 7, 9 male mice), h (n = 16, 13, 10, 14 male mice), i (n = 8, 7, 8, 8 male mice), j (n = 14, 12, 10, 12 male mice), k (n = 14, 12, 10, 12 male mice), l (n = 10, 12, 11, 7 male mice), m and n (n = 5 control-Gi and 9 Npy1R-Gi male mice). Boxplots indicate median ± min/max and include data points of individual mice entering the analysis. Statistical test: 2-way ANOVA followed by Tukey’s multiple a – n, except ordinary one-way ANOVA for l. Letters indicate statistical significance between: (a) Control vs. AgRP-Gq; (b) Control vs. POMC-Gi; (c) Control vs. AgRP-Gq;POMC-Gi. Source data and further details of statistical analyses are provided as a Source Data file.
