Extended Data Fig. 9: Scl-Ab alleviates memory impairment in AD mice with high serum sclerostin levels. | Nature Metabolism

Extended Data Fig. 9: Scl-Ab alleviates memory impairment in AD mice with high serum sclerostin levels.

From: Osteocyte-derived sclerostin impairs cognitive function during ageing and Alzheimer’s disease progression

Extended Data Fig. 9: Scl-Ab alleviates memory impairment in AD mice with high serum sclerostin levels.The alternative text for this image may have been generated using AI.

A. MicroCT analysis of the spongiosa of the femoral metaphysis. Quantification of the trabecular BS/BV, Tb.N, Tb.Sp, and Tb.Th (n = 8 WT-Ctrl mice, n = 8 AD-Ctrl mice, n = 8 AD rAAV-Sost mice, n = 6 AD rAAV-Sost+Scl-Ab mice). B. The levels of serum ALPL, Ocn, P1NP in mice from different groups (n = 5 WT-Ctrl mice, n = 5 AD-Ctrl mice, n = 4 AD rAAV-Sost mice, n = 5 AD rAAV-Sost+Scl-Ab mice). C. The protein levels of BACE1 and β-catenin in the cortex and hippocampus (2 biological replicates). D. Immunohistochemical staining for BACE1. E. Immunohistochemical staining for amyloid plaques using monoclonal anti-Aβ peptide (MOAB2) antibody. F-G. Analysis of athletic ability, anxiety and depression. Representative heat maps of the mice (F). Analysis of athletic ability, anxiety and depression, including the average speed, the time spent in the central area and the distance traveled in the central area (G) (n = 12 WT-Ctrl mice, n = 12 AD-Ctrl mice, n = 12 AD rAAV-Sost mice, n = 13 AD rAAV-Sost + Scl-Ab mice). H. The grey value analysis for the level of PSD95 and Syn in both cortex and hippocampus. Notes: WT-Ctrl: WT mice injected with rAAV9-DSS-Nter-Ctrl; AD-Ctrl: APP/PS1 mice injected with rAAV9-DSS-Nter-Ctrl; AD rAAV-Sost: APP/PS1 mice injected with rAAV9-DSS-Nter-Sost; AD rAAV-Sost+Scl-Ab: APP/PS1 mice injected with rAAV9-DSS-Nter-Sost and treated with Scl-Ab. Data are represented as mean ± SEM. One-way ANOVA followed by Turkey’s multiple comparisons test (A, B, G, H).

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