Fig. 1: Schematic overview of PMF-seq.
From: PMF-seq: a highly scalable screening strategy for linking genetics to mitochondrial bioenergetics
a, Schematic diagram of how substrates can feed into the respiratory chain to support the membrane potential. b, OCR of permeabilized A375 cells as measured by Seahorse analyser with glutamate/malate (left), succinate (middle) or ascorbate/TMPD (right) as the substrate. c, Kinetics of ΔΨm measured as TMRM fluorescence in permeabilized A375 cells with glutamate/malate (left), succinate (middle) or ascorbate/TMPD (right) as the substrate. d, Endpoint ΔΨm of permeabilized A375 cells measured as TMRM fluorescence by a flow cytometer with glutamate/malate (left), succinate (middle) or ascorbate/TMPD (right) as the substrate. e, Experimental workflow of PMF-seq in permeabilized A375 cells. IMS, intermembrane space; G/M, glutamate/malate; Succ, succinate; Pier, piericidin A; Anti, antimycin A; Oligo, oligomycin A; Bam, BAM15; CCCP, carbonyl cyanide m-chlorophenyl hydrazone; AU, arbitrary units.
