Extended Data Fig. 6: Additional data related to Fig. 4.
From: PMF-seq: a highly scalable screening strategy for linking genetics to mitochondrial bioenergetics
Transmission electron microscopy (TEM) images of A375 control cells (top) and ATPAF2 KO cells (bottom). Both control and ATPAF2 KO cells were permeabilized and subjected to treatment with either blank buffer (-tBID) or 100 nM tBID (+tBID), as indicated. Presented here are four distinct frames of view for each condition. Representative mitochondria with outer membrane breakage (red arrowhead), herniation (red arrow), or highly dilated cristae with interconnected intracristal space and contracted matrix (red asterisk) are noted. Scale bar = 600 nm. For each condition, at least 15 electron micrographs were collected, with four independent images being displayed.
