Extended Data Fig. 2: Genetic basis of branching at coenzyme Q.
From: PMF-seq: a highly scalable screening strategy for linking genetics to mitochondrial bioenergetics
a, Kinetics of mitochondrial membrane potential measured as TMRM fluorescence in permeabilized K562 cells with glycerol 3-phosphate (G3P) as the substrate. b, Endpoint mitochondrial membrane potential measured as TMRM fluorescence by a flow cytometer in permeabilized K562 cells with G3P as the substrate. c, Scatterplots of Z-scores of the specified respiratory chain components when succinate (first column), or glycerol 3-phosphate (second column) was used as the respiratory chain substrate in permeabilized K562 cells. Data from biological duplicates are shown. A highly negative Z-score indicates the enrichment of sgRNAs for a given gene in the low tail of the TMRM distribution, suggesting the dependency on that gene for membrane potential generation. d. Mean Z scores for GPD2 across screens. G3P, glycerol 3-phosphate; IMS, intermembrane space; G/M, glutamate/malate; Succ, succinate; Pier, piericidin A; Anti, antimycin A.
